Practice Test

Q1) Clonal cell lines can be obtained by: Show Answer

Q2) Which is non-invasive technique of genetic counselling? Show Answer

Q3) Who among the following discovered the enzyme restriction endonuclease? Show Answer

Q4) The first hormone artifically produced by culturing bacteria is: Show Answer

Q5) When the number of genes increase in response to some signal, the effect is called: Show Answer

Q6) A number of bacteria with recombinant DNA of same type form: Show Answer

Q7) Restriction enzyme may be used for: Show Answer

Q8) When scientists make an animal superior by view of genotype, introducing some foreign genes in it, the phenomenon is called: Show Answer

Q9) Many copies of a DNA molecule in a test tube are procured by: Show Answer

Q10) Genetic diagnosis by DNA testing: Show Answer

Q11) DNA can be introduced into any cell by: Show Answer

Q12) Bam HI, Eco RI, Sma H are the types of: Show Answer

Q13) The collection of bacteria with gDNA is called: Show Answer

Q14) Which one of the following pairs is correctly matched? Show Answer

Q15) Which one of the following scientists developed the process of DNA fingerprinting? Show Answer

Q16) DNA fingerprinting method is very useful for: Show Answer

Q17) In DNA fingerprinting: Show Answer

Q18) In Northern blotting RNAs are separated by gel electrophoresis and the RNA bands are transferred onto a membrane of: Show Answer

Q19) In Southern blotting is separated by gel electrophoresis: Show Answer

Q20) Transgenic animals are produced by injecting foreign gene into the: Show Answer

Q21) The process used for separation of protein in polyacrylamide gel is called: Show Answer

Q22) What is C-DNA? Show Answer

Q23) Which of the following organelles is associated with genetic engineering? Show Answer

Q24) The minimum length of cistron in base pairs which synthesizes a polypeptide of 50 amino acids is: Show Answer

Q25) The recent techniques used for separating fragments of DNA is: Show Answer

Q26) Plasmids are autonomously replicating mini chromosomes found in: Show Answer

Q27) In bacteria, genes for antibiotic resistance are usually located in: Show Answer

Q28) Which one of the following is related with genetic engineering? Show Answer

Q29) Improvement of genotype of an organism by addition of some foreign genes is: Show Answer

Q30) Two bacteria found to be very useful in genetic engineering experiments are: Show Answer

Q31) Restriction enzyme are isolated chiefly from: Show Answer

Q32) Which of the following statement is incorrect? Show Answer

Q33) The genetic recombinants obtained by insertion of plasmid into 1 phage genome is called: Show Answer

Q34) Synthetic DNA or sDNA is: Show Answer

Q35) A technique used to make numerous copies of a specific segment of DNA quickly and accurately: Show Answer

Q36) Human genome contains about: Show Answer

Q37) A collection of organisms, usually viruses, bacteria or yeast, which have been transformed by the addition of extra genes from another species: Show Answer

Q38) Colony hybridization procedure for identification of plasmid clones is called: Show Answer

Q39) TATAATG sequence near the RNA start point of prokaryotic promoter is: Show Answer

Q40) Which one of the following scientists got the Nobel Prize for his invention polymerase chain reaction (PCR)? Show Answer

Q41) An artificial process of infecting cells with naked viral DNA is: Show Answer

Q42) Producing a 'giant mouse' in the laboratory was possible through: Show Answer

Q43) One of the following is transgenic of organisms: Show Answer

Q44) An environmental agent that triggers transcription from an operon is a: Show Answer

Q45) Which of the following would not be used in preparing recombinant DNA? Show Answer

Q46) Ability to absorb foreign DNA is: Show Answer

Q47) RNA processing is: Show Answer

Q48) There are special proteins that help to open up DNA double helix infront of the replication fork. These proteins are: Show Answer

Q49) Who got the Noble prize in medicine for their discovery of 'G-proteins' and the role of these proteins in the cells: Show Answer

Q50) A gene is made up of: Show Answer

Q51) The message from nuclear DNA for the synthesis of specific cytoplasmic protein is carried by: Show Answer

Q52) Exonucleases cleaving nucleotides one at a time from the end of the polynucleotide chain are: Show Answer

Q53) Complete tranduction is: Show Answer

Q54) Inducible/lac operon system operates in: Show Answer

Q55) Nitrogen fixing genes are called: Show Answer

Q56) Genetic engineering is helpful in: Show Answer

Q57) Human Genome project was the thought of: Show Answer

Q58) The genome map was produced under human genome project in: Show Answer

Q59) Total genes in the human genome is approximately: Show Answer

Q60) Genes that are involved in turning on or off the transcription of a set of structural genes are called: Show Answer

Q61) Transgenic organisms are produced by: Show Answer

Q62) The basis for DNA fingerprinting is: Show Answer

Q63) Genetically bacteria have been successfully used in the commercial production of: Show Answer

Q64) Which of the following is associated with genetic engineering? Show Answer

Q65) PCR stands for: Show Answer

Q66) Enzyme used in PCR is: Show Answer

Q67) Yeast has become important in genetic engineering because it: Show Answer

Q68) Gene for cloning may be chemically synthesized: Show Answer

Q69) You are attempting to introduce a gene that imparts larval moth resistance to bean plants. Which of the following vectors are you most likely to use? Show Answer

Q70) Taq polymerase enzyme is found in: Show Answer

Q71) An attenuated virus: Show Answer

Q72) Which of the following is a difficulty in getting prokaryotic cells to express eukaryotic genes? Show Answer

Q73) Restriction endonucleases are used as: Show Answer

Q74) A somatic plant cell has potential to develop into a full plant. This is called: Show Answer

Q75) Enzymes breaking nucleic acids into nuleotides are called: Show Answer

Q76) Recombinant DNA technology is related with: Show Answer

Q77) Cosmid is: Show Answer

Q78) Match the correct one: Show Answer

Q79) Plasmids is used as carrier because: Show Answer

Q80) The function of polymerase chain reaction (PCR) is: Show Answer

Q81) Genetically engineered bacterium used in production of: Show Answer

Q82) Abnormal gene is replaced by normal gene through: Show Answer

Q83) In genetic engineering, the terms vector is applied for: Show Answer

Q84) Gene therapy involves: Show Answer

Q85) Which of the following enzyme is used in genetic engineering? Show Answer

Q86) The transgenic animals are those which have: Show Answer

Q87) Known sequence of DNA that is used to find complementary DNA strand is: Show Answer

Q88) Totipotency in cell is: Show Answer

Q89) The nuclease enzyme, which begins its attack from free end of a polynucleotide, is? Show Answer

Q90) Reverse transcriptase: Show Answer

Q91) Bovine spongiform encephalopathy disease is equal to: Show Answer

Q92) A clone of sheep Dolly has been made by: Show Answer

Q93) Which one of the following bacteria has found extensive use in genetic engineering work in plants? Show Answer

Q94) Variable number of tandem repeats (VTNRs) in the DNA molecule are highly useful in: Show Answer

Q95) The first cloned animal of the world is: Show Answer

Q96) In transgenics, the expression of transgene in the target tissue is known by: Show Answer

Q97) DNA fingerprinting is related to: Show Answer

Q98) Which of the following is specifically used in genetic engineering? Show Answer

Q99) Molecular scissors, which cut DNA at specific site: Show Answer

Q100) Plasmids are extrachromosomal circular DNA molecules: Show Answer

Q101) Identify the plasmid: Show Answer

Q102) Natural genetic engineer is: Show Answer

Q103) The most thoroughly studied of the known bacteria-plant interaction is the: Show Answer

Q104) What does Bt stand for the popular crop Bt cotton? Show Answer

Q105) DNA fingerprinting techniques was first developed by: Show Answer

Q106) What is the first step in Southern Blot technique? Show Answer

Q107) An example of gene therapy is: Show Answer

Q108) The total number of nitrogenous bases in human genome is estimated to be about: Show Answer

Q109) Name of the drug used in cancer treatment produced by using biotechnology is: Show Answer

Q110) Which of the following pairs is correctly matched? Show Answer

Q111) Somaclonal variation can be obtained by: Show Answer

Q112) The enzyme used for cutting DNA segment in genetic engineering is: Show Answer

Q113) Widely used tool in genetic engineering of crop plants is: Show Answer

Q114) Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Show Answer

Q115) There is a restriction endonuclease called EcoRI. What does "co" part in it stand for? Show Answer

Q116) Agarose extracted from sea weeds finds use in: Show Answer

Q117) In DNA fingerprinting which of the following is true? Show Answer

Q118) Matching sequence of DNA between two evidences, one of the criminal with the suspect is known as: Show Answer

Q119) A technology which has found immense use in solving cases of disputed parentage is: Show Answer

Q120) First hormone prepared by genetic engineering is: Show Answer

Q121) First biochemical to be produced commercially by microbial cloning and genetic engineering is: Show Answer

Q122) c-DNA probes are copied from the messenger RNA molecules with the help of: Show Answer

Q123) Which one of the following is a correct statement Show Answer

Q124) A tumour inducing plasmid widely used in the production of transgenic plants in that of: Show Answer

Q125) Common bacterium used in genetic engineering is: Show Answer

Q126) Find the incorrect statement: Show Answer

Q127) Two microbes found to be very useful in genetic engineering are: Show Answer

Q128) What is the function of Restriction endonuclease? Show Answer

Q129) A genetically engineered micro-organism used successfully bioremediation of oil spills is a species of: Show Answer

Q130) The construction of the first recombinant DNA was done by using the native plasmid of: Show Answer

Q131) The basis of DNA fingerprinting is: Show Answer

Q132) The linking of antibiotic resistance gene with the plasmid vector became possible with: Show Answer

Q133) Gel electrophoresis is used for: Show Answer

Q134) Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? Show Answer

Q135) An improved variety of transgenic basmati rice: Show Answer

Q136) Genetic engineering has been successfully used for producing: Show Answer

Q137) The genetically-modified (GM) brinjal in India has been developed for: Show Answer

Q138) Genetic engineering considered to be a branch of biotechnology is concerned with: Show Answer

Q139) Which of the following is used as carrier of transferring of selected DNA into cells? Show Answer

Q140) Which of the following restriction endonuclease enzymes produce blunt end? Show Answer

Q141) Which of the following is correct with regard to recombinant of DNA technology? Show Answer

Q142) Restriction endonucleases: Show Answer

Q143) Plasmids: Show Answer

Q144) Which of the following properties makes plasmids suitable vectors for gene cloning? Show Answer

Q145) Plasmids present in the bacterial cells are: Show Answer

Q146) The first step in genetic engineering is: Show Answer

Q147) Hybridisation through protoplast fusion is known as: Show Answer

Q148) The term humulin is used for: Show Answer

Q149) Synthesis of DNA can be stopped by incorporating artificial: Show Answer

Q150) The technique to detect specific genes is called: Show Answer

Q151) Which of the following statement is incorrect? Show Answer

Q152) Restriction enzymes are used in genetic engineering because Show Answer

Q153) Process by which we can add or delete certain gene is Show Answer

Q154) Which type of bond is formed by the sticky ends with their complementary counterparts? Show Answer

Q155) Since DNA has a ______ charge, it moves towards the _____ electrode of the electrophoretic chamber Show Answer

Q156) What must be done before placing DNA into the electrophoretic chamber? Show Answer

Q157) How many major types of restriction endonucleases have been recongnised? Show Answer

Q158) X technique is now routinely used to detect HIV in suspected AIDS patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. Identify X Show Answer

Q159) Which of the following is known as chemical knife of DNA? Show Answer

Q160) The type of bond formed by DNA ligase between adjacent nucleotides is Show Answer

Q161) Which statement is wrong? Show Answer

Q162) The procedure through which a piece of DNA is introduced in a host bacterium is called Show Answer

Q163) Which of the following has the ability to transform normal cells into cancerous cells in animals? Show Answer

Q164) Which of the following is not applicable to Agrobacterium tumifaciens? Show Answer

Q165) Insertional inactivation is related to Show Answer

Q166) For transformation with recombinant DNA, the bacterial cells must first be made 'competent' which means Show Answer

Q167) Which of the following method can be used for making the bacterial cell 'competent'? Show Answer

Q168) Which of the following techniques can be used to introduce foreign DNA into cell? Show Answer

Q169) During heat shock to the bacterium, the temperature used for giving thermal shock is Show Answer

Q170) Which of the following enzyme is used in case of fungus to cause release of DNA along with other macromolecules? Show Answer

Q171) During isolation of DNA, addition of which of the following causes precipitation of purified DNA? Show Answer

Q172) Which of the following is the correct sequence of PCR or polymerase chain reaction? Show Answer

Q173) The most commonly used bioreactor is of stirring type. The stirrer facilitates Show Answer

Q174) Which of the following palindromic sequence is recognize by EcoRI? Show Answer

Q175) During gel electrophoresis for separation of DNA fragment Show Answer

Q176) Fill up the blanks -
A. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of _____ and these can be joined together end-to-end using ______.
B. Disruption of the cell membranes can be achieved by treating the bacterial cells plant or animal tissue with enzymes such as ______ (bacteria), _____ (plant cells) and ______ (fungus).
C. If any protein encoding gene is expressed in a heterologous host, it is called a _____.
D. In gel electrophoresis, the separated DNA fragments can be seen only after staining the DNA with a compound known as ________ followed by exposure to UV radiation. Show Answer

Q177) Fill up the blanks -
A. The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native _____ of _____.
B. Hind II always cut DNA molecules at a particular point by recognizing a specific sequence of ______ base pairs. This specific sequence is known as _____ for Hind II.
C. The ______ in DNA is a sequence of base that reads same on the two strands when orientation of reading is kept the same.
D. ______, a pathogen of several dicot plants is able to deliver a piece of DNA known as ______ to transform normal plant cells into tumor cells. Show Answer

Q178) Identify the true statements -
A. The first recombinant DNA was constructed by using a piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of E. coli.
B. Cohen and Boyer are known as father of genetic engineering.
C. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligases.
D. Endonucleases remove nucleotides from the ends of the DNA whereas exonucleases make cuts at specific positions within the DNA.
E. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
F. Humulin was the first recombinant DNA, based product, produced and marketed in India.
G. YAC vectors contain the telomeric sequence the centromere and autonomously replicating sequence from yeast chromosomes.
H. Alkaline phosphatase, is used to prevent unwanted self ligation of the vector DNA molecules in procedures of DNA technology.
I. pBR322 vector was the first artificial ideal vector constructed by Boliver and Rodriguer.
J. Plasmid DNA is coated with histone proteins and can act as genetic factor. Show Answer

Q179) Pairing of fragments derived from DNA is a process called Show Answer

Q180) The controlled use of biological agents, such as micro-organisms or cellular components, for beneficial use is called Show Answer

Q181) The experimental manipulation of DNA of different species, producing recombinant DNA is known as Show Answer

Q182) Which of the following is used as cloning vehicle? Show Answer

Q183) The tumour-inducing capacity of Agrobacterium tumifacines is located in large extrachromosomal plasmids called Show Answer

Q184) Which enzyme is used as gum to join ends of DNA? Show Answer

Q185) Which one of the following restriction endonuclease is obtained from Escherichia coli? Show Answer

Q186) A bioreactor (fermenter) refers to Show Answer

Q187) The first restriction endonuclease reported was Show Answer

Q188) Which one is incorrect? Show Answer

Q189) DNA fragments can be separated by a technique known as Show Answer

Q190) T-DNA is found in Show Answer

Q191) Which of the following methods are used to introduce alien DNA Show Answer

Q192) Which enzymes are used to break the cell to release DNA? Show Answer

Q193) Which enzyme is used to join cut DNA? Show Answer

Q194) Taq DNA polymerase enzyme is obtained from Show Answer

Q195) If a protein encoding gene is expressed in a heteroolgous host it is called Show Answer

Q196) Which of the following are required to facilitate cloning into a vector? Show Answer

Q197) Which is incorrect? Show Answer

Q198) Which of the following has been used as cloning vector? Show Answer

Q199) A device in which substances are treated to stimulate transformation by living cells is called Show Answer

Q200) Which of the following has helped in development of biotechnology? Show Answer

Q201) Which is not true for stirred tank fermentation? Show Answer

Q202) Which statement is incorrect? Show Answer

Q203) During DNA purification, which enzyme is used to treat plant cells? Show Answer

Q204) Restriction endonucleases enzymes are used in genetic engineering because they Show Answer

Q205) Which of the following is/are part(s) of biotechnology?
A. in vitro fertilization
B. synthesis of a gene
C. correcting a defective gene
D. developing a DNA vaccine Show Answer

Q206) Which of the following is/are used in recombinant DNA technology?
A. agarose gel
B. ethidium bromide
C. plasmid vector
D. restriction endonuclease Show Answer

Q207) Which of the following vectors are used to deliver a desirable gene into animal and plant cells, respectively? Show Answer

Q208) Which of the method(s) is/are used to introduce foreign DNA into host cells? Show Answer

Q209) Due to ampicillin resistance gene, one is able to select a transformed cell in the presence of ampicillin. The ampicillin resistance gene in this case is called Show Answer

Q210) Which of the following statements are true about scientist Paul Berg?
A. he is the Father of genetic engineering
B. he transferred gene of SV 40 virus into E. Coli
C. he got the Nobel Prize in chemistry in 1980
D. he developed hybridoma technology for the production of monoclonal antibiotics Show Answer

Q211) What are the properties of a good vector?
A. it should be ideally more than 10 kb in size
B. it should be able to replicate autonomously
C. it should have suitable marker genes
D. it should not be easy to isolate and purify Show Answer

Q212) Gene gun or particle bombardment gun method of gene transfer utilizes Show Answer

Q213) The areas of application of PCR include
A. production of monoclonal antibodies
B. insertion of recombinant DNA into organism
C. diagnosis of specific mutation
D. detection of plant pathogens Show Answer

Q214) After the formation of the product in the bioreactors, it undergoes through separation and purification processes before a finished product is ready for marketing. These processes are collectively referred to as Show Answer

Q215) Which one is associated with downstream processing?
A. Separation
B. Purification
C. DNA isolation
D. Gene expression Show Answer

Q216) Which of the following restriction enzymes produces blunt ends? Show Answer

Q217) Which of the following is/are concerned with biotechnology? Show Answer

Q218) Restriction endonucleases -
A. are found in bacteria
B. are very useful in genetic engineering
C. cut the DNA at particular sites
D. are used naturally in a bacterial cell to defend against foreign DNA Show Answer

Q219) DNA recombinant technology uses -
A. Restriction endonucleases
B. DNA ligase
C. Cloning vector
D. Electrophoresis Show Answer

Q220) Genetic engineering is Show Answer

Q221) Which of the following is not a tool used to perform genetic engineering Show Answer

Q222) PCR needs Show Answer

Q223) A. Large scale production involves use of bioreactors.
B. Genetic engineering involves the use of restriction endonuclease, DNA ligase appropriate plasmid or viral vectors.
C. Modern biotechnology uses genetically modified organisms.
D. Bacterial cell wall is digested by lysozyme. Show Answer

Q224) A. In elution the separated bands of DNA are cut from agarose gel and extracted from the gel piece.
B. E. coli cloning vector pBR322 shows several restriction, Ori, antibiotic resistance genes and Rop.
C. The down stream processing and quality control testing vary from produced to product.
D. Competent bacterial cell cannot take up the plasmid. Show Answer

Q225) Which statement is incorrect?
A. Genetic engineering is also called recombinant DNA technology.
B. Bacteriophage is not used as vector.
C. MALAYALAM is a palindrome.
D. Ethidium bromide can not be used for staining DNA. Show Answer

Q226) Which one is associated with Agrobacterium tumifaciens?
A. Tumor
B. Ti plasmid
C. T-DNA
D. Cancerous cells Show Answer

Q227) Which steps are involved in genetically modifying an organism?
A. Identification of desirable DNA
B. Insertion of DNA into the host
C. Maintenance of introduced DNA in the host
D. Isolation of recombinant protein Show Answer

Q228) Which statement is true about bioreactor?
A. Bioreactor provides the optimal conditions for obtaining the desired product
B. Raw materials are biologically converted into specific products
C. A stirred-tank reactor is horizontal in shape
D. Large volume of cultures cannot be processed Show Answer

Q229) Restriction endonucleases hydrolyzes polynucleotide from Show Answer

Q230) Most common reporter gene whose product can be directly visualized in transformed cells is Show Answer

Q231) For a plasmid to be a cloning vector, the minimum numbers of elements required are Show Answer

Q232) Which of the following is incorrect about reporter gene Show Answer

Q233) DNA of a bacterium is not cleaved by its own restriction enzymes because the recognition DNA sequences are Show Answer

Q234) Agrobacterium tumefaciens is an effective vector for use with Show Answer

Q235) The first nuclear transplant from an animal cell into an enucleated egg that produced a normal offspring was performed in Show Answer

Q236) Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then Show Answer

Q237) A mouse in which one particular gene has been replaced by its inactivated form generated in vitro is called Show Answer

Q238) Which of the following tools of recombinant DNA technology is incorrectly paired with its use? Show Answer

Q239) In recombinant DNA methods, the term vector refers to Show Answer

Q240) Which of the following is not component of yeast artificial chromosome Show Answer

Q241) Restriction endonucleases are enzymes that -
P. cleave the 5' terminal nucleotides from duplex DNA molecules
Q. make sequence specific cuts in both strands of duplex DNA molecules
R. promote circularization of the duplex DNA molecule by removal of the 5' terminal nucleotides
S. generate 3'-hydroxyl and 5'-phosphate ends in the cut DNA strands Show Answer

Q242) Elution is a method applied for Show Answer

Q243) A plant genetic engineer wishes to transfer and express a gene from sunflower into beans. Which of the following would be the vector of choice? Show Answer

Q244) Terminal transferase is used Show Answer

Q245) Which statement about restriction enzymes are false? Show Answer

Q246) Restriction-modification systems of bacteria exists to Show Answer

Q247) Which of the following is a desirable characteristic for a cloning plasmid? Show Answer

Q248) Eukaryotic genes may not function properly when cloned into bacteria because of Show Answer

Q249) Restriction endonucleases are Show Answer

Q250) Which statement correctly describes sequential steps in cDNA cloning? Show Answer

Q251) The following are useful to introduce genes into crop plants except Show Answer

Q252) Which one of these statements about the applications of gene cloning is false? Show Answer

Q253) Cosmid vectors are used for constructing libraries of DNA fragments of eukaryotes because they Show Answer

Q254) Which of the following is not true about pBR322 vector? Show Answer

Q255) The presence of a plasmid in a bacterial culture is usually determined by Show Answer

Q256) Which of the following processes require energy? Show Answer

Q257) To be a cloning vector, a plasmid does not require Show Answer

Q258) In agarose gel electrophoresis Show Answer

Q259) Shotgun approach is used for the construction of Show Answer

Q260) pBR322 which is frequently used as a vector for cloning gene in E. coli is Show Answer

Q261) Select the correct option which shows the most appropriate temperatures of these different steps of PCR mechanism Show Answer

Q262) Which of the following statement is not correct? Show Answer

Q263) Which of the following statement is not correct about cloning vector Show Answer

Q264) Which of the following statement is not correct Show Answer

Q265) Which of the following is the part of biotechnology Show Answer

Q266) European Federation of Biotechnology (FEB) has given a definition of biotechnology that Show Answer

Q267) The enzymes responsible for restricting the growth of bacteriohage in 'Escherichia coli' is Show Answer

Q268) Which of the following statement is not true Show Answer

Q269) The most common tool of genetic engineering Show Answer

Q270) Antibiotic resistance gene present on BamHI site of a E. coli cloning vector is Show Answer

Q271) Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because Show Answer

Q272) In E. coli cloning vector PBR322. ROP codes for Show Answer

Q273) The bacteria associated with plant genetic engineering are Show Answer

Q274) Direct visual selection method for the selection of recombinant host cells on the basis of their inability to produce colour in the presence of chromogenic substrate can be applied if the vector used is a Show Answer

Q275) Which of the following vectors is/are used for cloning in eukaryotic organisms?
A. Plasmids
B. Bacteriophages
C. Ti plasmid of Agrobacterium tumifaciens
D. Disarmed retroviruses Show Answer

Q276) Which of the following features cannot be associates with Ti plasmid of Agrobacterium tumifaciens which is modified into a cloning vector? Show Answer

Q277) If recombinant DNA is inserted within the coding sequence of enzyme galactosidase, which of the following will occur in case of non-recombinants? Show Answer

Q278) Following enzymes/techniques are used in the process of recombinant DNA technology -
A. EcoRI to cut the isolated genome
B. DNA ligase
C. Protease and ribonuclease for removal of proteins and RNA from DNA
D. Production of reombinant hosts
E. Lysozyme for isolation of the genetic material (DNA)
F. Gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use Show Answer

Q279) In case of pBR322, which of the following act as selectable markers? Show Answer

Q280) After completion of biosynthetic stage, the product has to be subjected through a series of processes before it is ready for marketing as finished product. This series of processes is called Show Answer

Q281) Agrobacterium tumefaciens -
A. Acts as natural genetic engineer
B. Carries vie region which is essential for insertion of T-DNA into host cells
C. Has been used to transfer genes for 'RNA interference' in plants
Mark the correct statements Show Answer

Q282) Continuous addition of sugars in 'fed batch' fermentation is done to Show Answer

Q283) Read the following four statements (A-D) about certain mistakes in two of them -
A. The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched
B. Restriction enzymes are used in isolation of DNA from other macro-molecules
C. Downstream processing is one of the steps of R-DNA technology
D. Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes? Show Answer

Q284) Biolistics (gene-gun) is suitable for Show Answer

Q285) In genetic engineering, the antibiotics are used Show Answer

Q286) Cohen and Boyer isolated an antibiotic resistance gene, by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance, in the year Show Answer

Q287) Restriction enzyme EcoRI cuts the DNA between bases G and A only when the sequence in DNA is Show Answer

Q288) Amplification of gene of interest by using DNA polymerase may to upto Show Answer

Q289) Which of the statement is incorrect? Show Answer

Q290) A single strand of nucleic acid tagged with a radioactive molecule is called Show Answer

Q291) Which one of the following is a case of wrong matching Show Answer

Q292) For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of Show Answer

Q293) Which of the following is not correctly matched for the organism and its cell wall degrading enzyme? Show Answer

Q294) Blue-white selection is used Show Answer

Q295) An analysis of chromosomal DNA using the Southern hybridization technique does not use Show Answer

Q296) Which vector can clone only a small fragment of DNA? Show Answer

Q297) Identify the desirable characteristics for a plasmid used in rDNA technology from the following -
A. Ability to multiply and express outside the host in a bioreactor
B. A highly active promoter
C. A site at which replication can be initiated
D. One or more identifiable marker genes
E. One or more unique restriction sites Show Answer

Q298) In order induce the bacterial uptake of plasmids, the bacteria are made competent by first treating with Show Answer

Q299) The sticky ends on each of the two strands of a DNA generated by treatment with restriction enzyme facilitate the action of enzyme Show Answer

Q300) In pBR322, the gene sequence that codes for the proteins involved in the replication of the plasmid is Show Answer

Q301) Selective markers in plasmids are used to Show Answer

Q302) If the transformed bacterial cells are selected by blue/white screening, it means that the reporter enzyme/protein in this case is Show Answer

Q303) The best cloning vector to transfer large fragment of passenger DNA into host cell is Show Answer

Q304) In the year ___X____, the two enzymes responsible for restricting the growth of bacteriohphage in ___Y____ were isolated. One of these added methyl groups to DNA, while the other cut DNA.
Choose the option which correctly fills up the blanks X and Y is Show Answer

Q305) Consider the given statements w.r.t. gel electrophoresis -
I. DNA can be separated by forcing them to move towards cathode under an electric field
II. DNA fragments separate according to their size through sieving effect provided by agarose gel
III. The used matrix in this technique is agarose which is an artificial polymer
Which of the following statements is/are correct? Show Answer

Q306) Select the wrong statement Show Answer

Q307) The cloning vector M13 has genetic material Show Answer

Q308) In the nomenclature of enzyme restriction endonuclease the Roman numerical indicates Show Answer

Q309) One of the methods by which DNA cannot be transferred to the host cell is by Show Answer

Q310) The DNA molecule to which the gene of interest is integrated for cloning is called Show Answer

Q311) The introduction of T-DNA into plants involves Show Answer

Q312) The cutting of DNA at specific locations became possible with the discovery of Show Answer

Q313) Which of the following is not a feature of the plasmids? Show Answer

Q314) The taq polymerase enzyme is obtained from Show Answer

Q315) Stirred-tank bioreactors have been designed for Show Answer

Q316) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using Show Answer

Q317) Which of the following is not a component of downstream processing? Show Answer

Q318) The uptake of naked DNA by bacteria is called Show Answer

Q319) The DNA-joining enzyme, required in recombinant DNA technology is Show Answer

Q320) Isolation of DNA from a fungal cell involves the use of enzyme Show Answer

Q321) Statement 1: All endonuclease cut DNA at specific sites
Statement 2: Endonucleases are found in viruses Show Answer

Q322) Statement 1: Restriction endonucleases are also called ‘molecular scissors’
Statement 2: When fragments generated by restriction endonucleases are mixed, they join together
due to their sticky ends Show Answer

Q323) Statement 1: Agrobacterium tumfaciens is popular in genetic engineering because this bacterium is
associated with roots of all cereals and pulse crops
Statement 2: A gene incorporated in the bacterial chromosomal genome gets automatically transferred
to the crop with which the bacterium is associated Show Answer

Q324) Statement 1: In recombinant DNA technology, human genes are often transferred into bacteria or yeast
Statement 2: Both bacteria and yeast multiply very fast to form huge population which expresses the
desired gene Show Answer

Q325) Retroviruses in animals including humans are able to change normal cells into Show Answer

Q326) pBR322 was the first artificial cloning vector developed in …A… by …B… and …C… from E. coli plasmid.
Here A, B and C can be Show Answer

Q327) Transfer of any gene into a completely different organism can be done through Show Answer

Q328) Recombinant DNA have integrated fragment of Show Answer

Q329) In plants, the tumour inducing plasmid (Ti) of Agrobacterium tumefaciens is used as a cloning vector.
This statement is Show Answer

Q330) If recombinant DNA carrying antibiotic resistance (e.g., ampicillin) is transferred into E. coli cell, the host
cell is transformed into ampicillin-resistant cells. The ampicillin resistant gene in this case is called a Show Answer

Q331) Proteins are removed by treatment with Show Answer

Q332) Which of the following key factors, makes plasmid, the vector in genetic engineering? Show Answer

Q333) I.Ori also controls the copy numbers of the linked DNA
II. If a foreign DNA ligates at the Bam HI site of tetracycline resistance gene in the vector pBR322, the
recombinant plasmid loses the tetracycline resistance due to insertion of foreign DNA
Choose regarding the above statements Show Answer

Q334) Downstream process includes
I. Separation of the product from the reactor
II. Purification of the product
III. Formation of the product with suitable preservatives
IV. Quality control testing and clinical trials in case of drugs
Which of the statements given above are correct? Show Answer

Q335) More advancement in genetic engineering is due to Show Answer

Q336) Plasmid are suitable vectors for gene cloning because Show Answer

Q337) Polymerase chain reaction is useful in Show Answer

Q338) Which of the following techniques can be used to detect genetic disorders in human? Show Answer

Q339) Primers are Show Answer

Q340) This method of finding a gene is used when researchers very little about the gene they are trying to find.
This process results in a complete gene library : a collection of copies of DNA fragments that represent the entire genome of an organism. Identify the method Show Answer

Q341) Consider the following statement about PCR
I. Polymerase Chain Reaction (PCR) is a technique of synthesizing multiple copies of the desired gene in
vitro
II. This technique was developed by Kary Mullis in 1985
III. A single PCR amplification cycle involves three basic steps; denaturation, annealing and extension
Which of the statement given above are correct? Show Answer

Q342) Ori is a DNA sequence that is responsible for initiating replication. This statement is Show Answer

Q343) Plasmids are autonomously replicating circular extrachromosomal DNA. This statement is Show Answer

Q344) A single PCR amplification cycle involves Show Answer

Q345) Which of the following are used to gene cloning? Show Answer

Q346) The process that preserves the distribution of DNA fragments in the gel while creating replica on the filter
is one of the following Show Answer

Q347) Two enzymes responsible for restricting the growth of bacteriophages in E. coli were isolated. One was
methylase and other was restriction endonuclease. What is the significance of methylase? Show Answer

Q348) Single-stranded DNA molecules that can bind to and be used to detect other DNA molecules are called Show Answer

Q349) The controlled use of biological agents, such as microorganism, plants or animal cell, for beneficial use is
called Show Answer

Q350) Consider the following statements
I. In microinjection method foreign DNA is directly injected into the nucleus of animal cell or plant cell by
using micro needles or micro pipettes
II. Microinjection method is used in oocytes, eggs and embryo
III. Electroporation is the formation of temporary pores in the plasma membrane of host cell by using
lysozyme or calcium chloride
IV. In chemical mediated gene transfer method certain chemicals such as CO2 help foreign DNA to enter
the host cell
Which of the statements given above are correct? Show Answer

Q351) Polyethylene glycol method is used for Show Answer

Q352) The enzymes, commonly used in genetic engineering are Show Answer

Q353) Which one of the following techniques had helped to solve many mysteries involving murders, robberies
and rapes? Show Answer

Q354) Consider the following statements
I. Recombinant DNA technology popularly known as genetic engineering is a stream of biotechnology
which deals with the manipulation of genetic material by man in vitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriquez from E.coli
plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases
Which of the statements given above are correct? Show Answer

Q355) PCR was developed by …A… in …B… and for this he received Nobel Prize for chemistry in …C… . Here A, B
and C can be recognized as
Show Answer

Q356) Cutting of a piece of DNA from a plasmid was done with the help of …A… enzymes, popularly known as
…B… . Here A and B can be Show Answer

Q357) In a genetic engineering experiment, restriction enzymes can be used for Show Answer

Q358) The components of a bioreactor are
I. an agitator system
II. an oxygen delivery system
III. foam control system
IV. temperature control system
V. pH control system
VI. sampling ports to with draw cultures periodically
Choose the correct option Show Answer

Q359) I. DNA being a hydrophilic molecule cannot pass through cell membranes
II. The bacteria should be made competent to accept the DNA molecule
The correct option regarding the above statements is Show Answer

Q360) In cloning plasmid pBR322
p stands for …A…
B stands for …B…
R stands for …C…
Choose the correct option Show Answer

Q361) Blood stains are found at the site of murder. If DNA profiling technique is to be used for identifying the
criminal, which of the following is ideal for use? Show Answer

Q362) Which of the following is required to perform polymerase chain reaction?
I. DNA template
II. Primer
III. Taq polymerase and vent polymerase
Choose the correct option Show Answer

Q363) In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector.
Consider the following four agents (I-IV) in this regard and select the correct option about which one or
more of these can be used as a vector/vectors
I. Bacterium
II. Plasmid
III. Plasmodium
IV. Bacteriophage Show Answer

Q364) Thermostable enzymes ′taq′ and ′ventDNA polymerase′ isolated from thermophilic bacteria are Show Answer

Q365) Due to chloramphenicol resistance gene, one is able to select a transformed cell in the presence of
chloramphenicol. The chloramphenicol resistance gene in this case is called Show Answer

Q366) GAATTC is the recognition site for the restriction endonuclease Show Answer

Q367) Plasmid is Show Answer

Q368) The polymerase chain reaction is a technique that is used for Show Answer

Q369) Enzyme that is used in PCR technology is Show Answer

Q370) An enzyme catalyzing the removal of nucleotides from the ends of DNA is Show Answer

Q371) Polymerase Chain Reaction (PCR) needs Show Answer

Q372) Consider the following statements
I. A soil inhabiting plant bacterium, Agrobacterium tumefaciens , a pathogen of several dicot plants is
able to transfer a piece of DNA known as T-DNA
II. The T-DNA causes tumours
III. Tumour formation induced by Ti-plasmid
Which of the statements given above are correct? Show Answer

Q373) Restriction endonucleases are enzymes which Show Answer

Q374) Which of the following is correctly matched? Show Answer

Q375) The Polymerase Chain Reaction (PCR) is a reaction in which amplification of specific DNA sequences is
carried out in vitro. This statement is Show Answer

Q376) Restriction enzymes was discovered by Show Answer

Q377) Consider the following statements
I. Bioreactors are vessels of large volumes in which raw materials are biologically converted into specific
products
II. One of the most commonly used bioreactors is of stirring type
III. Shake flasks are used for growing and mixing the desired materials on a small scale in the laboratory
IV. A large scale production of desired biotechnological product is done by using ‘bioreactors’ Show Answer

Q378) The term ‘Biotechnology’ was given by Show Answer

Q379) Which of the following statement is not true?
I. DNA being a hydrophilic molecule cannot pass through cell membranes
II. Agrobacterium tumefaciens delivers a piece of DNA known as ‘Z-DNA’ in the Ti-plasmid which
transforms normal plant cells into tumour cells to produce chemical against pathogens
III. Retrovirus, adenovirus, papillomavirus are also now used as cloning vectors in animal because of their
ability to transform normal cells into cancerous cell.
IV. In genetic engineering, DNA from different sources are cut with the same restriction enzymes so that
both DNA fragments have same kind of sticky ends
Choose the correct option Show Answer

Q380) PCR technique was invented by Show Answer

Q381) The tumour inducing capacity of Agrobacterium tumefaciens is located in large extrachromosomal
plasmids called Show Answer

Q382) Who discovered recombinant DNA (rDNA) technology? Show Answer

Q383) Which of the following is used in recombinant DNA technique? Show Answer

Q384) For selectable marker.
I. It helps to select the host cells which contain the vector and eliminate the non transformants
II. Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline or kanamycin, are
useful selectable markers for E. coli
Which of the statements given above are correct? Show Answer

Q385) Who discovered that restriction enzymes have the capability of cutting DNA strands in a particular
fashion, which left what has became known as ‘sticky ends’ on the strands? Show Answer

Q386) A restriction fragment containing a specific gene of interest can be identified by gel electrophoresis
followed by transferring the DNA to a membrane as a solid support matrix using a procedure called Show Answer

Q387) About gene gun method
I. This method is also known as biolistic technique
II. In this method cells are bombarded with high velocity micro-particles of gold or tungsten coated with
DNA in plants
III. Important crop plants like maize, rice and wheat have now been transformed by this method
Which of the statements given above are correct? Show Answer

Q388) RNA is removed by the treatment with Show Answer

Q389) Process of formation of RNA from DNA is called Show Answer

Q390) Which of the following components are used in gel electrophoresis?
I. Ethidium bromide
II. Restriction endonuclease
III. Agarose
IV. UV radiation
Choose the correct option Show Answer

Q391) Microorganisms can be grown in the bioreactor by Show Answer

Q392) Which one of the following is commonly used in transfer of foreign DNA into crop plants? Show Answer

Q393) There …A… are the DNA molecules that can carry a foreign …B… segment into the host cell.
Here A and B refers to
Show Answer

Q394) Probes, used in DNA fingerprinting are initially Show Answer

Q395) Application of PCR are
I. detection of pathogens
II. diagnosis of specific mutation
III. DNA fingerprinting
Choose the correct option Show Answer

Q396) The role of DNA ligase in the construction of a recombinant DNA molecule is Show Answer

Q397) Biolistics (gene gun) is suitable for Show Answer

Q398) Which of the following statements are correct for the enzyme taq polymerases?
I. Taq polymerase is thermally unstable
II. It requires primers for carrying out the process of polymerization
III. Taq polymerase is isolated from thermophilic bacterium, Thermus aquaticus
Choose the correct option Show Answer

Q399) EFB stands for Show Answer

Q400) The commonly used DNA fingerprinting technique in forensic studies is simply a method involving Show Answer

Q401) Cry I endotoxins obtained from Bacillus thruigiensis are effective against Show Answer

Q402) In the naming of restriction enzymes the first letter is derived from …A… name and next two letters from
the …B… and fourth letter from …C… of …D… where the enzymes are extracted
A to D in the statement can be
Show Answer

Q403) Which of the following techniques is most commonly used to separate DNA molecules by size? Show Answer

Q404) Which of the following steps are catalyzed by taq polymerase in a PCR reaction? Show Answer

Q405) I. In the process of recombinant DNA technology after several treatment the purified DNA is precipitated
by adding chilled ethanol
II. The bacterial/plant, animal cell is broken down by enzymes to release DNA, along with RNA, proteins,
polysaccharides and lipids
Choose the correct option for above statements Show Answer

Q406) Which of the statements are correct about bioreactors?
I. It provides all the optimal conditions for achieving the desired product by providing optimal growth
conditions like temperature, pH, substrate, salt, vitamin and oxygen
II. It is suited for large-scale production of microorganisms under aseptic conditions for a number of days
Correct option is Show Answer

Q407) Taq polymerase enzyme used in PCR is isolated from Show Answer

Q408) The first restriction endonuclease type II …A…, was isolated by Smith, Wilcox and Kelley from …B…
bacterium. It was formed to cut DNA molecules at a particular point by recognizing a specific sequence of
six base pairs, known as the …C… . Here A, B and C can be
Show Answer

Q409) In gel electrophoresis, the separated DNA fragments are visualized after staining the DNA with …A…
followed by exposure to …B…
Here A and B refers to
A B Show Answer

Q410) Following enzymes/chemical/technique are used in the process of gel electrophoresis
I. sample DNA is cut into fragments
II. restriction endonucleases
III. agarose gel
IV. ethidium bromide
V. UV-radiation
VI. elution
Mark the correct sequence of their use Show Answer

Q411) Which one is a true statement regarding DNA polymerase used in polymerase chain reaction? Show Answer

Q412) Most sensitive technique to detect malignant cell in non-hodgkin’s lymphoma is Show Answer

Q413) Which of the following methods(s) is used to introduce foreign DNA into host cells? Show Answer

Q414) After completion of the biosynthetic stage in the bioreactors, the product undergoes. Separation and
purification processes, collectively termed as Show Answer

Q415) Which of the following should be choosen for best yield if one has to produce a recombinant protein or
enzyme on a large scale, using microbial plants/anima/human cell? Show Answer

Q416) Which of the following is known as molecular scissors of DNA? Show Answer

Q417) A kind of biotechnology involving manipulation of DNA is Show Answer

Q418) Polymerase chain reaction employs Show Answer

Q419) An antibiotic resistance gene in a vector usually helps in the selection of Show Answer

Q420) Which of the following statements are correct with respect to a bioreactor?
I. It can process small volume of culture
II. It provides optimum temperature, pH, salt, vitamins and oxygen
III. Sparged stirred-tank bioreactor is a stirred type reactor in which air is bubbled
Choose the correct option Show Answer

Q421) Vent polymerase enzyme used in PCR is isolated from Show Answer

Q422) Stirred-tank bioreactors have advantages over shake flasks because they Show Answer

Q423) During ‘gene cloning’ which is called a gene taxi? Show Answer

Q424) I. Copy number is defined as the number of copies of plasmid present in a cell
II. It varies from 15-100 copies per cell
Choose regarding the above statements Show Answer

Q425) Which of the following has popularized the PCR (polymerase chain reaction)? Show Answer

Q426) The first recombinant DNA was constructed by Show Answer

Q427) Source of taq polymerase used in PCR is a Show Answer

Q428) Which of the following is used as a best genetic vector in plants? Show Answer

Q429) Plants in comparison to animals are more rapidly manipulated by genetic engineering. Select out the most
probable reason for this Show Answer

Q430) In recombinant DNA technique, the term vector refers to a Show Answer

Q431) The steps involved in the Southern blot test are as follows
I. X-ray film
II. Electrophoresis
III. Digestion with restriction enzyme
IV. Ethidium bromide
V. Radioactive probe
Choose the option having correct sequential order of these events Show Answer

Q432) Find out the wrong statements Show Answer

Q433) The mobile genetic element is Show Answer

Q434) Identify the palindromic sequence in the following Show Answer

Q435) The different basic steps of genetic engineering are given below randomly
I. Identification of DNA with desirable genes
II. Gene transfer
III. Maintenance of DNA in host and gene cloning
IV. Introduction of DNA into host to from recombinant DNA
Which of the following represents the correct sequence of steps? Show Answer

Q436) Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct
order
I. Extraction of the desired gene product
II. Amplification of the gene of interest
III. Isolation of a desired DNA fragment
IV. Ligation of the DNA fragment into a vector
V. Insertion of recombinant DNA into the host
Correct order is Show Answer

Q437) I. …A… is the ability of a cell to take up foreign DNA
II. The cell is treated with specific concentration of a divalent cation such as …B… to increase pore size in
cell wall
III. In ….C… method recombinant DNA is directly injected into the nucleus of an animal cell
The most appropriate option regarding A, B and C is Show Answer

Q438) The most important feature in a plasmid to be used as a vector is Show Answer

Q439) DNA gyrase, the enzyme that participates in the process of DNA replication is a type of Show Answer

Q440) The key tools required for the recombinant DNA technology are
I. restriction enzymes II. Polymerase enzymes
III. host organism ligases IV. Vectors
V. host organisms
Select the correct option Show Answer

Q441) Which of the following infection (s) can be diagnosed by the use of polymerase chain reaction? Show Answer

Q442) Agarose is extracted from Show Answer

Q443) Which one is a true statement regarding DNA polymerase used in PCR? Show Answer

Q444) The two main techniques that gave birth to modern biotechnology are
I. chemical engineering
II. genetic engineering
III. human genome engineering
IV. molecular biology
Choose the correct option Show Answer

Q445) Which is incorrect statement? Show Answer

Q446) Which of the following would have the highest oxygen transfer rate characteristics? Show Answer

Q447) Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still
attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be
analysed, the best way of getting sufficient amount of DNA from this extract is Show Answer

Q448) The characteristics of a molecular probe are
I. very long molecule
II. double-stranded
III. DNA or RNA
IV. complementary to a part of desired gene
The correct pair is Show Answer

Q449) VNTR analysis involves Show Answer

Q450) Manipulation of DNA in genetic engineering became possible due to the discovery of Show Answer

Q451) Alec Jeffreys developed the DNA fingerprinting technique. The probe he used was Show Answer

Q452) In addition to taq polymerase enzyme which other thermostable DNA polymerases have been isolated to
be used in Polymerase Chain Reaction (PCR)? Show Answer

Q453) PCR proceeds in three distinct steps governed by temperature. They are in order of Show Answer

Q454) In gel electrophoresis, the separated bands of DNA are cut out and extracted from the gel piece. This step
is called Show Answer

Q455) Significance of heat shock method in bacterial transformation is facilitate Show Answer

Q456) Minisatellite or Variable Number Tendem Repeat (VNTR) are used in Show Answer

Q457) Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where
would you find the smallest segment of DNA? Show Answer

Q458) The structure involved in genetic engineering is Show Answer

Q459) In agarose gel electrophoresis, DNA molecules are separated on the basis of their Show Answer

Q460) In gel electrophoresis, the sample DNA is cut into fragments by Show Answer

Q461) In case of polymerase chain reaction, temperature, required for the steps
A. Denaturation
B. Annealing
C. Extension Show Answer

Q462) The experimental manipulation of DNA of different species, producing recombination DNA is known as Show Answer

Q463) Which of the following statement is correct in the context of observing DNA separated by agarose gel
electrophoresis? Show Answer

Q464) Using recombinant technology, genes from a donor cell can be transplanted into a bacterium for DNA
replication and protein synthesis. The kinds of cells that can be used as a donor in this technology are Show Answer

Q465) Transformation is defined as the procedure by which a piece of …A… is introduced into a …B… host. Here A
and B refers to
Show Answer

Q466) The restriction enzyme responsible for the cleavage of following sequence is
5′ − G − T − C − G − A − C − 3′
3′ − C − A − G − C − T − G − 5′ Show Answer

Q467) Boviene spongiform encephalopathy disease is equal to: Show Answer

Q468) Many copies of a DNA molecule in a test tube are produced by: Show Answer

Q469) Special sequence in the DNA recognized by restriction endonuclease is called Show Answer

Q470) Genetic engineering is possible because: Show Answer

Q471) The basic of DNA fingerprinting is: Show Answer

Q472) Gene amplification using primers can be done by Show Answer

Q473) The genome of Caenorhabditis elgans consists of: Show Answer

Q474) Then linking of antibiotic resistance gene with the plasmid vector became possible with: Show Answer

Q475) Restriction enzymes are used to cut Show Answer

Q476) Restriction enzymes are isolated chiefly from: Show Answer

Q477) What is the first step in Southern Blotting technique? Show Answer

Q478) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant
bacteria because of: Show Answer

Q479) The first hormone artificially produced by culturing bacteria is: Show Answer

Q480) Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What
is so special shown in it?
5’_________GAATTC___________3’
3’_________CTTAAG___________5’ Show Answer

Q481) The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called Show Answer

Q482) The nuclease enzyme, which beings its attack from free end of a polynucleotide, is? Show Answer

Q483) In Southern blotting……… is separated by gel electrophoresis: Show Answer

Q484) Harris and J.F. Watkins in 1965 first time reported the fusion of following cell lines to form hybrids: Show Answer

Q485) PCR and Restriction Fragment Length Polymorphism are the methods for: Show Answer

Q486) Restriction enzymes may be used for: Show Answer

Q487) Production of a human protein in bacteria in genetic engineering is possible because: Show Answer

Q488) Petroleum-lysing bacteria are being engineering for the removal of oil spills. What is the most realistic
danger of these bacteria to the environment? Show Answer

Q489) Complete transduction is: Show Answer

Q490) The restriction enzyme(s) used in recombinant DNA technology that make staggered cuts in DNA leaving
sticky ends is/are Show Answer

Q491) When the number of genes increases in response to some signal, the effect is called: Show Answer

Q492) DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by: Show Answer

Q493) There is a restriction endonuclease called Eco RI. What does “co” part in it stand for? Show Answer

Q494) Enzymes breaking nucleic acids into nucleotides are called: Show Answer

Q495) Manipulation of gene and genetic material by man is a fast emerging branch of science which started with the formation of recombinant DNA molecules. This branch of science is named as Show Answer

Q496) For transformation, microparticles coated with DNA are to be bombarded with gene gun are made up of: Show Answer

Q497) Simple stirred-tank bioreactor is given below. Identify A,B,C,D and E Show Answer

Q498) Plasmid is used as carrier because: Show Answer

Q499) Variable number of tendem repeats (VTNRs) in the DNA molecule are highly useful in: Show Answer

Q500) DNA fingerprinting technique was first developed by: Show Answer